ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3802
Quantity100 reactions

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Western diet-induced obesity results in brain mitochondrial dysfunction in female Ossabaw swineFront Mol NeurosciDecember 14, 2023Taylor J. Kelty, Chris L. Taylor, Nicole E. Wieschhaus, Pamela K. Thorne, Amira R. Amin, Christina M. Mueller, T. Dylan Olver, Darla L. Tharp, Craig A. Emter, Alexander W. Caulk, et al.per manufacturer’s instructions (Qiagen, Cat#74104) and a cDNA library was synthesized (Promega, Cat#A3802). A nanodrop spectrometer was used to assess RNA and cDNA purity. Quantitative real-time PCR (qRT-PCR)

(2) Defining diurnal fluctuations in mouse choroid plexus and CSF at high molecular, spatial, and temporal resolutionNature CommunicationsJune 22, 2023Ryann M. Fame, Peter N. Kalugin, Boryana Petrova, Huixin Xu, Paul A. Soden, Frederick B. Shipley, Neil Dani, Bradford Grant, Aja Pragana, Joshua P. Head, et al.reverse-transcribed into cDNA using the Promega™ ImProm-II™ Reverse Transcription System (Fisher PR-A3802) following manufacturer’s specifications with random hexamer primers (Promega PR-C1181). RT-qPCRs were

(3) Primary cilia sense glutamine availability and respond via asparagine synthetaseNature metabolismMarch 1, 2023Steidl ME, Nigro EA, Nielsen AK, Pagliarini R, Cassina L, Lampis M, Podrini C, Chiaravalli M, Mannella V, Distefano G, et al.(Promega, #C1101) or Random Primers (Promega, #C1181) and ImProm-II Reverse Transcriptase (Promega, #A3802). Quantitative real-time PCR analysis was performed on technical duplicates using iTaq Univer SYBR Green

(4) Transcriptional expression of CXCL10 and STAT1 in lupus nephritis and the intervention effect of triptolideClinical rheumatologyFebruary 1, 2023Shi D, Li Y, Shi X, Yao M, Wu D, Zheng Y, Lin Q, Yang Y.from Japan Tongren Institute of Chemistry. The RNA extraction kit(#LS1040), reverse transcription kit(#A3802), and real-time fluorescence quantitative PCR(#A1250) kit were purchased from Promega Company. The upstream

(5) Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibitionNeuronJuly 6, 2022Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P.Oligo(dT)15 primer (Promega C1101) for flanking primer analysis and ImProm II reverse transcriptase (Promega A3802). For qPCR assays, two technical replicates were run per experiment and the mean was calculated. The

(6) Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro StudiesFrontiers in MicrobiologyMay 10, 2022Rajkumar Kulandaisamy et al.per manufacturer's instructions to synthesise the cDNA using ImProm-II Reverse Transcriptase (Cat # A3802, Promega Corporation, India). One-fourth of the cDNA preparation and the primer set, MBA134 (Forward)

(7) Impairment of Glucose Metabolism and Suppression of Stemness in MCF-7/SC Human Breast Cancer Stem Cells by NootkatonePharmaceuticsApril 21, 2022Yen Thi-Kim Nguyen, Ngoc Bao To, Vi Nguyen-Phuong Truong, Hee Young Kim, Meran Keshawa Ediriweera, Yoongho Lim, Somi Kim Cho(Invitrogen) reagent and reversed-transcribed into cDNA using ImProm-IITM Reverse Transcriptase (cat. no. A3802; Promega, Madison, WI, USA) following the manufacturer’s instructions. Then, two-step quantitative real-time PCR

(8) Spatiotemporal analysis identifies ABF2 and ABF3 as key hubs of endodermal response to nitrateProceedings of the National Academy of SciencesJanuary 25, 2022Orlando Contreras-López et al.using ImProm-II Reverse Transcription System according to the instruction of the manufacturer (Catalog #A3802, Promega). qRT-PCR was carried out using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix following

(9) Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesisCell reportsDecember 28, 2021Méndez-Solís O, Bendjennat M, Naipauer J, Theodoridis PR, Ho JJD, Verdun RE, Hare JM, Cesarman E, Lee S, Mesri EA.79254 Amplification grade DNase I,Sigma,Cat.# AMPD1 Im-PromII Reverse Transcriptase,Promega,Cat.# A3802 Lipofectamine 2000,Life Technologies,Cat.# 11668-027 Lipofectamine RNAiMAX reagent,Life Technologies,Cat.#

(10) Role of laminin and cognate receptors in cholangiocarcinoma cell migrationCell adhesion & migrationDecember 1, 2021Islam K, Thummarati P, Kaewkong P, Sripa B, Suthiphongchai T.

(11) SPX-related genes regulate phosphorus homeostasis in the marine phytoplankton, Phaeodactylum tricornutumCommunications BiologyDecember 1, 2021Kaidian Zhang, Zhi Zhou, Jiashun Li, Jingtian Wang, Liying Yu, Senjie Lin

(12) CHD4 Conceals Aberrant CTCF-Binding Sites at TAD Interiors by Regulating Chromatin Accessibility in Mouse Embryonic Stem CellsMolecules and cellsNovember 30, 2021Han S, Lee H, Lee AJ, Kim SK, Jung I, Koh GY, Kim TK, Lee D.DNase-treated total RNA was applied for cDNA synthesis using an Improm-II Reverse transcription system (A3802; Promega, USA) according to the manufacturer’s protocol. For analysis of retrotransposon expression,

(13) Analysing the fitness cost of antibiotic resistance to identify targets for combination antimicrobialsNature MicrobiologyNovember 1, 2021Aviram Rasouly et al.total RNA to cDNA was performed with the ImProm reverse transcriptase system (Promega, cat. number: A3802). As a proxy for the rate of rRNA synthesis, the level of the short-lived 5’ end of the ribosomal RNA

(14) Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's diseaseScientific reportsOctober 8, 2021Prasad R, Jung H, Tan A, Song Y, Moon S, Shaker MR, Sun W, Lee J, Ryu H, Lim HK, et al.TRIzol reagent (Invitrogen) and cDNA synthesis was performed using ImProm‐II™ Reverse Transcriptase (#A3802, Promega) according to the manufacturer's protocol. Quantitative PCR (qPCR) was carried out using CFX

(15) A New Role for Plastid Thioredoxins in Seed Physiology in Relation to Hormone RegulationInternational Journal of Molecular SciencesSeptember 27, 2021Guillaume Née, Gilles Châtel-Innocenti, Patrice Meimoun, Juliette Leymarie, Françoise Montrichard, Pascale Satour, Christophe Bailly, Emmanuelle Issakidis-Bourguetstep was performed with up to 480 ng total RNA, using the ImProm-II™ Reverse Transcription System #A3802 (Promega, Charbonnières, France) with oligo(dT) primer. Gene expression profiling was conducted by real-time

(16) The sugar-responsive circadian clock regulator bZIP63 modulates plant growthThe New phytologistSeptember 1, 2021Viana AJC, Matiolli CC, Newman DW, Vieira JGP, Duarte GT, Martins MCM, Gilbault E, Hotta CT, Caldana C, Vincentz M.1.5 µg total RNA (final volume 12.5 ml) was performed using ImProm II Reverse Transcriptase (cat. no. A3802; Promega) and oligo(dT)18 according to the manufacturer’s instructions. qRT‐PCR analyses were performed

(17) Stability of hepatitis B virus pregenomic RNA in plasma specimens under various temperatures and storage conditionsPeerJApril 14, 2021Pakkapon Rattanachaisit, Sirinporn Suksawatamnuay, Supachaya Sriphoosanaphan, Kessarin Thanapirom, Panarat Thaimai, Nipaporn Siripon, Sukanya Sittisomwong, Yong Poovorawan, Piyawat Komolmitinstructions. Isolated HBV RNA was reverse transcribed using ImProm-II Reverse Transcriptase (Cat No. A3802; Promega, Madison, WI, USA), with HBV-specific RT primers (5-ATTCTCAGACCGTAGCACACGACACCGAGATTGAGATCTTCTGCGAC-3)

(18) Cx3cr1CreERT2-driven Atg7 deletion in adult mice induces intestinal adhesionMolecular BrainDecember 1, 2020Younghwan Lee, Ji-Won Lee, Hyeri Nam, Seong-Woon YuKit (Qiagen, 73,404). cDNA was synthesized using the ImProm-II Reverse Transcriptase kit (Promega, A3802) and oligo-dT primers. TOPreal qPCR 2× PreMIX (SYBR Green with low ROX; Enzynomics, RT500M) containing

(19) Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cellsScientific ReportsDecember 1, 2020Einar Vargas-Bello-Pérez, Nathaly Cancino-Padilla, Carolina Geldsetzer-Mendoza, María Sol Morales, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor, Jaime RomeroMadison, USA), in the amount of 0.5 µL, and 1 µL ImProm-II Reverse Transcriptase (Promega, Cat. No. A3802, Madison, USA) was added. Following addition of transcription mix, the reaction was maintained at 25

(20) Insulin signaling and glucose metabolism in different hepatoma cell lines deviate from hepatocyte physiology toward a convergent aberrant phenotypeScientific ReportsDecember 1, 2020Angela Molinaro, Barbara Becattini, Giovanni Solinasguanidinium-thiocyanate method. The complementary DNA was prepared using reverse transcription kit (Promega A3802). qPCR was performed using SYBR green mix (Bio-Rad 172-5274) and the following primers: mouse G6P fwd

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